Dibutyl Phthalate Adsorbed on Multiwalled Carbon Nanotubes Causes Fetal Developmental Toxicity in Balb/C Mice

This study investigated whether using multiwalled carbon nanotubes (MWCNTs) as a carrier for dibutyl phthalate (DBP) could delay the degradation rate of DBP in mice and increase its estrogen-like interference effect. Pregnant Balb/C were divided into four groups exposed to different treatments via tail-vein injection every 3 days until gestational day 20. The female male then sacrificed toxicological study. results showed that combination MWCNTs resulted higher fetal mortality than if or alone. H&E staining estrous period was delayed, development oocytes blocked group, number spermatogenic cells decreased, quality sperm decreased. Our experiment expression levels genes involved sex hormone synthesis testis ovaries significantly increased after combined treatment compared with MWCNT group (p < 0.01). suggests is delayed when absorbed on MWCNTs, which increases interferes development, ultimately leading mortality.